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SOUZA,EVERTON H.; SOUZA,FERNANDA V.D.; ROSSI,MÔNICA L.; PACKER,RENAN M.; CRUZ-BARROS,MARIA AMELIA V.; MARTINELLI,ADRIANA P.. |
ABSTRACT Pollen morphology characterization is important in taxonomy, conservation and plant breeding, and pollen viability studies can support breeding programs. This study investigated pollen morphology and male fertility in 18 species of Bromeliaceae with ornamental potential. For morphological characterization, pollen grains were acetolyzed and characterization of exine was done using scanning and transmission electron microscopy. Pollen viability was investigated by in vitro germination and histochemical tests. Species belonging to Aechmea and Ananas genera presented medium size pollen, except for Ae. fasciata, with large pollen. Al. nahoumii, P. sagenarius and the Vriesea species analyzed showed large pollen, except for V. carinata, with very large... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aechmea; Alcantarea; Ananas; In vitro pollen germination; Histochemical test; Vriesea. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000603067 |
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Soares,Taliane Leila; Souza,Everton Hilo de; Costa,Maria Angélica Pereira de Carvalho; Silva,Sebastião de Oliveira e; Santos-Serejo,Janay Almeida dos. |
ABSTRACT Obtaining banana tetraploid cultivars from triploid strains results in total or partial reestablishment of fertility, allowing the occurrence of some fruits with seeds, a feature that is undesirable from a marketing perspective. The objective of this study was to assess the viability of pollen of 12 banana tetraploid hybrids (AAAB) by means of in vitro germination and two histochemical tests (acetocarmine and 2,3,5-triphenyltetrazolium chloride). The pollen tube growth was evaluated by germinating grains in three culture media — M1: 0.03% Ca(NO3)∙4H2O, 0.02% Mg(SO4)∙7H2O, 0.01% KNO3, 0.01% H3BO3 and 15% sucrose; M2: 0.03% Ca(NO3)∙4H2O, 0.01% KNO3, 0.01% H3BO3 and 10% sucrose; and M3: 0.015% H3BO3, 0.045% Ca3(PO4)2 and 25% sucrose. The acetocarmine... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa L.; In vitro pollen germination; Histochemical analysis; Plant breeding. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000200145 |
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